Sarcoma Cell Line Resource with Molecular and Kinase Annotation
The aim of this application is to create a sarcoma cell line resource that will be readily available to academic and industry laboratories. We will establish a molecular cytogenetic profile for each of 30 sarcoma cell lines, and will validate that the lines are faithful biologically to the sarcomas from which they were originally established. Further, we will use a proteomic phosphokinase screening method to determine whether the cell lines contain potential therapeutic targets. These data will be integrated with cDNA array profiles from Matt van de Rijn (see separate application), and the entire panel of cell lines will then be deposited with the American Type Culture Collection (ATCC) for public distribution. The proposed studies represent a substantial first step in providing a comprehensive panel of sarcoma cell lines to the research community.